Course Description: The primary objective of this course is to familiarize students with the process of preparing tissue samples for histological examination, which holds significant importance in contemporary medical diagnosis. Additionally, the course aims to provide students with hands-on experience in working with human tissues in a laboratory setting, teaching them the necessary procedures for accurate diagnosis and effective treatment approaches.
Requirements for enrollment of courses:
The mandatory prerequisites for enrolling in the course include having attended the classes for Histology and Embryology Courses.
Anticipated Learning Outcomes for the Subject:
Upon successful completion of the course, students are expected to grasp the significance of specific histological techniques used in contemporary laboratory diagnostics, essential for the functioning of clinical medicine. Their prior knowledge of the microscopic structure of the human body and expertise in presenting individual substances and antigens will foster a deeper comprehension of pathomorphological alterations.
Acquiring knowledge of histological laboratory procedures, tissue preparation techniques for analysis, and contemporary methods of displaying individual tissues, cells, and antigens, including molecules on both the cell surface and inside cells, will enhance the ability to identify diverse diagnostic requirements encountered in clinical medicine.
Subject Contents: The course covers the following topics:
- Preparation and Processing of Samples in the Histological Laboratory:
- Detailed procedures involving materials, chemicals, and laboratory utensils.
- Paraffin Preparation of Histological Preparations:
- Techniques for embedding tissue samples in paraffin for sectioning and staining.
- Preparation of Frozen Preparations:
- Techniques for freezing tissue samples for immediate sectioning and examination.
- Classic Histological Staining:
- Overview of traditional staining methods to visualize tissue structures.
- Histochemical Techniques:
- Methods for demonstrating specific chemical substances in tissues.
- Immunohistochemical Techniques:
- Various immunofluorescent and immunoenzymatic techniques for detecting antigens in tissues.
- Direct and indirect staining methods.
- Signal amplification techniques.
- Antigen retrieval techniques.
By the end of the course, students will have a comprehensive understanding of these subject contents, enabling them to proficiently work in a histological laboratory and effectively utilize these techniques in clinical medicine for diagnostic purposes.